GenScript LApro Taq DNA Polymerase possesses both 3’->5’ proofreading activity and 5’->3’ DNA polymerase activity optimized to amplify longer GC-rich fragments.
GenScript LApro Taq DNA Polymerase possesses both 3->5 proofreading activity and 5->3 DNA polymerase activity optimized to amplify longer GC-rich fragments. As one of the most thermostable DNA polymerases known to have 3>5 proofreading activity, the enzyme catalyzes DNA synthesis at anoptimal temperature near 75℃ with very low error rate (it is about ten times more accurate than standard Taq DNA polymerase). The enzyme is optimized to achieve amplification of 17.5 kb products from human genomic DNA and 20 kb from λDNA. Using LApro Taq DNA Polymerase in long PCR* amplifications can reduce smearing and virtually eliminate unwanted background artifacts. LApro Taq DNA Polymerase can generate the A-ended PCR fragments required for T/A cloning.
Key Features: High capacity: One unit of enzyme can manage up to 20 kb from human genomic DNA. High fidelity Increased stability: LaPro Taq DNA Polymerase is more stable than standard Taq DNA polymerase.
Some of the applications of LApro Taq DNA Polymerase are as follows:
- Up to 20kb from λDNA
- High fidelity
- More stable than normal Taq DNA Polymerase
- TA cloning
- Site-directed mutagenesis
- Fragment analysis
- LApro Taq DNA Polymerase
- 1.5ml, 2x buffer, 500 mM Tris-HCl (pH 8.9 at 25 ℃), 0.5 % Tween 20, 0.5% Nonidet P40, 1 mg/ml BSA ,with 3 mM MgCl2
- dNTP,10 mM 50ul
- Control template and primers for 25 reactions
LApro Taq DNA Polymerase shows very good fidelity and an average error rate around 2x10-6. LApro Taq DNA Polymerase has 5->3 exonuclease activity and 3->5 proofreading activity. It also has the extendase activity allowing TA cloning.
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74 ℃.
Storage Buffer and Concentration:
Supplied in 5 units/μl in 50 mM Tris HCl (pH 8.1), 0.1 mM EDTA, 1 mM DTT, 0.1% (v/v) Nonidet P40, 0.1% (v/v) Tween 20 and 50% (v/v) glycerol.
Store at -20 ℃. The enzyme will remain stable for at least one year, if stored properly.