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MKK6 (Phospho-Ser207) pAb Blocking Peptide

MKK6 (Phospho-Ser207) pAb Blocking Peptide

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详细说明

Catelog Peptide:MKK6 (Phospho-Ser207) pAb Blocking Peptide(RP19789)The synthesized phosphopeptide was derived from human MKK6 around the phosphorylation site of serine 207 (V-D-SP-V-A).

Full Name
MKK6 (Phospho-Ser207) pAb Blocking Peptide  
Description Catelog Peptide:MKK6 (Phospho-Ser207) pAb Blocking Peptide(RP19789)The synthesized phosphopeptide was derived from human MKK6 around the phosphorylation site of serine 207 (V-D-SP-V-A). 
Concentration 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol. 
Quality Control The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. 
Applications

For Western blotting: add 10 ?l of antibody and 10 ?l of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 ?l of antibody and 2 ?l of blocking peptide to 0.096 ml of buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the slides.

 
Specificity The peptide specifically blocks the signal of Rabbit Anti MKK6 (Phospho-Ser207) completely in western blotting and immunohistochemistry.  
storage Store at -20℃