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IKK α (Ab-23) pAb Blocking Peptide

IKK α (Ab-23) pAb Blocking Peptide

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详细说明

Catelog Peptide:IKK α (Ab-23) pAb Blocking Peptide(RP19750)The synthesized non-phosphopeptide was derived from human IKKα around the phosphorylation site of Threonine 23 (L-G-TP-G-G).

Full Name
IKK α (Ab-23) pAb Blocking Peptide  
Description Catelog Peptide:IKK α (Ab-23) pAb Blocking Peptide(RP19750)The synthesized non-phosphopeptide was derived from human IKKα around the phosphorylation site of Threonine 23 (L-G-TP-G-G). 
Concentration 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol. 
Quality Control The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. 
Applications

For Western blotting: add 10 ?l of antibody and 10 ?l of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 ?l of antibody and 2 ?l of blocking peptide to 0.096 ml of buffer, and incubate at 4℃ over night or at room temperature for 2 hours before allowing to react with the slides.

 
Specificity The peptide specifically blocks the signal of Rabbit Anti IKKα (Ab-23) completely in western blotting and immunohistochemistry.  
storage Store at -20℃