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Caspase-8 Colorimetric Assay Kit

Caspase-8 Colorimetric Assay Kit

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详细说明

The Caspase-8 Colorimetric Protease Assay Kit (Cat. No. L00303) provides a simple and convenient means of assaying the activity of caspase-8. The activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells.

Description:

The Caspase-8 Colorimetric Protease Assay Kit (Cat. No. L00303) provides a simple and convenient means of assaying the activity of caspase-8. The activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells. The assay for caspase-8 is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-8 activity can be determined.

Key features:

  • Simplified Procedure: The components in this kit are provided ready-to-use.
  • Supreme Reliability: The kit produces highly reproducible results.
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