IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin.
| Full Name | | Insulin Like Growth Factor-1 (IGF-1) (4-70), human | |
| Abbreviated Name-1 | rHu IGF-I(4-70), Recombinant Human IGF-I (4-70) |
| Abbreviated Name-2 | Recombinant Insulin-Like Growth Factor-I (4-70) |
| Description | IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.
GenScript Insulin Like Growth Factor (IGF)-1 (4-70), human , produced in E. coli, is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7,372 Da. |
| Source | E. coli |
| M.W. | 7,372 Da |
| Purity | The purity of GenScript Recombinant Human IGF is greater than 95% as determined by the following methods:
(a) HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
| Endotoxin Level | The endotoxin level of GenScript Recombinant Human IGF is below 0.1 ng/μg (1 IEU/μg) of IGF-I (4-70). |
| Storage | Although lyophilized GenScript IGF-I (4-70) can remain stable for three weeks if stored at room temperature, it is best stored desiccated below -18°C. After reconstitution, IGF-I (4-70) may be stored at 4°C for up to seven days and below -18°C for longer periods. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Please avoid freeze-thaw cycles. |
| Formulation | IGF-I (4-70) is lyophilized after extensive dialysis against acetic acid buffer. |
| Reconstitution | It is recommended that lyophilized IGF-I (4-70) be stored in 100 mM acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Specificity | The ED50 of GenScript Recombinant Human IGF, as calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less than 1.0 ng/ml, corresponding to a specific activity of 1.0×106 IU/mg. For most in vitro applications, GenScript IGF-I (4-70) exerts its biological activity in the concentration range of 0.2-20 ng/ml. |