Insulin Like Growth Factor-1 (IGF-1) (4-70), human

Full Name

Insulin Like Growth Factor-1 (IGF-1) (4-70), human

Abbreviated Name-1

rHu IGF-I(4-70), Recombinant Human IGF-I (4-70)

Abbreviated Name-2

Recombinant Insulin-Like Growth Factor-I (4-70)

Description

IGF is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.
GenScript Insulin Like Growth Factor (IGF)-1 (4-70), human , produced in E. coli, is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7,372 Da.

Source

E. coli

M.W.

7,372 Da

Purity

The purity of GenScript Recombinant Human IGF is greater than 95% as determined by the following methods:
(a) HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE silver-stained gel analysis

Endotoxin Level

The endotoxin level of GenScript Recombinant Human IGF is below 0.1 ng/μg (1 IEU/μg) of IGF-I (4-70).

Storage

Although lyophilized GenScript IGF-I (4-70) can remain stable for three weeks if stored at room temperature, it is best stored desiccated below -18°C. After reconstitution, IGF-I (4-70) may be stored at 4°C for up to seven days and below -18°C for longer periods. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Please avoid freeze-thaw cycles.

Formulation

IGF-I (4-70) is lyophilized after extensive dialysis against acetic acid buffer.

Reconstitution

It is recommended that lyophilized IGF-I (4-70) be stored in 100 mM acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specificity

The ED50 of GenScript Recombinant Human IGF, as calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less than 1.0 ng/ml, corresponding to a specific activity of 1.0×10⁶ IU/mg. For most in vitro applications, GenScript IGF-I (4-70) exerts its biological activity in the concentration range of 0.2-20 ng/ml.

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Insulin Like Growth Factor-1 (IGF-1) (4-70), human