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Interleukin-2 (IL-2), human

Interleukin-2 (IL-2), human

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In vitro studies performed on human cell lines demonstrate the immunoregulatory properties of IL-2 including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human IL-2 dependent edll lines; 2) enhancement of lymphocyte cytotoxicity;

Full Name
Interleukin-2 (IL-2), human  
Abbreviated Name-1 rHuIL-2; rHuIL2
Abbreviated Name-2 Interleukin-2; Interleukin2
Description In vitro studies performed on human cell lines demonstrate the immunoregulatory properties of IL-2 including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human IL-2 dependent edll lines; 2) enhancement of lymphocyte cytotoxicity; 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity; 4) induction of interferon-gamma production.
Interleukin (IL)-2, human is a highly purified protein with a molecular weight of approximately 15,300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the human IL-2 gene, and the resulting expression clone encodes a modified human IL-2. This recombinant form differs from native interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.  
M.W. 15,300 Da  
Purity Greater than 95% as determined by the following methods:
(a) SEC-HPLC analysis
(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
Endotoxin Level Less than 0.1 ng/μg (1 IEU/μg) determined by LAL test  
Specific Activity The specific activity as determined and was found to be greater than 1.0×107 IU/mg.  
Storage Lyophilized rHuIL-2 remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution rHuIL-2 should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.  
Formulation The protein (1.1 mg/ml) was lyophilized after extensive dialysis against 0.17 mg sodium monobasic and 0.89 mg dibasic sodium phosphate buffer to a pH7.5.  
Reconstitution It is recommended to reconstitute the lyophilized rHuIL-2 in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. 
Sequence analysis The sequence of the first five N-terminal amino acids has been found to be Met-Pro-Thr-Ser-Ser.