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Anti-Bovine PKC-Biotin

Anti-Bovine PKC-Biotin

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详细说明

Anti-Bovine Protein Kinase C (PKC) BiotinMonoclonal AntibodyProd. No.: P108 Clone: MC5 Isotype: Mouse IgG2a Conc.: 0.1 mg/ml Pkg. Size: 0.25 mg Storage: 2-8°C

Anti-Bovine Protein Kinase C (PKC) Biotin
Monoclonal Antibody
Prod. No.:  P108
Clone:      MC5
Isotype:    Mouse IgG2a
Conc.:      0.1 mg/ml
Pkg. Size:  0.25 mg
Storage:    2-8°C

Description
Specificity:
Anti-Protein Kinase C (PKC) recognizes the intact (Mr 79-80 kDa) calcium-activated, phospholipid-dependent Protein Kinase C in bovine brain. The antibody strongly cross-reacts with PKC in human, mouse and rat cells.1 Anti-PKC (clone MC5) binds to an epitope at the hinge region of the enzyme, the site of phorbol-ester-induced proteolysis which is thought to down-regulate PKC activity in intact cells.1,2 Monoclonal MC5 reacts with PKC-α/ß1/ß2 isoforms.

Background:
Anti-PKC-Biotin (clone MC5) may be used to block both proteolysis and down-regulation of PKC activity. The antibody reacts with the intact molecule and may be used to localize PKC activity and to study the role of proteolysis in the activation of the Protein Kinase C pathway.

Known Species Cross Reactivity: Ms+, Hu+, Rt+, Rb+, Bov+
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (Frozen), WB
Western Blotting: Leinco Technologies suggests a working dilution of approximately 1:100 for western blotting or other immunochemical techniques. However, each investigator should determine their own optimal working dilution for each specific research application.

References
1. Young, S. et al. (1988) Eur. J. Biochem. 172:247
2. Young, S. et al. (1987) Biochem. J. 244:775
Related Products
Anti-Bovine PKC Protein Kinase C Purified (Leinco Prod. No.: P107)
Anti-Bovine PKC Agarose (Leinco Prod. No.: P110)
Anti-Bovine PKC-Fluorescein (FITC) (Leinco Prod. No.: P109)

Products are for research use only. Not for use in diagnostic or therapeutic procedures