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 Anti-Bovine PKC-Biotin
  • Anti-Bovine PKC-Biotin
  • Anti-Bovine PKC-Biotin

    参考价格: ¥0.00
    适用范围:全领域
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    上架时间:2011年12月16日
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    详细说明

    Anti-Bovine Protein Kinase C (PKC) BiotinMonoclonal AntibodyProd. No.: P108 Clone: MC5 Isotype: Mouse IgG2a Conc.: 0.1 mg/ml Pkg. Size: 0.25 mg Storage: 2-8°C

    Anti-Bovine Protein Kinase C (PKC) Biotin
    Monoclonal Antibody
    Prod. No.:  P108
    Clone:      MC5
    Isotype:    Mouse IgG2a
    Conc.:      0.1 mg/ml
    Pkg. Size:  0.25 mg
    Storage:    2-8°C

    Description
    Specificity:
    Anti-Protein Kinase C (PKC) recognizes the intact (Mr 79-80 kDa) calcium-activated, phospholipid-dependent Protein Kinase C in bovine brain. The antibody strongly cross-reacts with PKC in human, mouse and rat cells.1 Anti-PKC (clone MC5) binds to an epitope at the hinge region of the enzyme, the site of phorbol-ester-induced proteolysis which is thought to down-regulate PKC activity in intact cells.1,2 Monoclonal MC5 reacts with PKC-α/ß1/ß2 isoforms.

    Background:
    Anti-PKC-Biotin (clone MC5) may be used to block both proteolysis and down-regulation of PKC activity. The antibody reacts with the intact molecule and may be used to localize PKC activity and to study the role of proteolysis in the activation of the Protein Kinase C pathway.

    Known Species Cross Reactivity: Ms+, Hu+, Rt+, Rb+, Bov+
    Host Species
    Mouse
    Formulation
    This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
    Storage and Stability
    This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
    Reported Applications
    IHC (Frozen), WB
    Western Blotting: Leinco Technologies suggests a working dilution of approximately 1:100 for western blotting or other immunochemical techniques. However, each investigator should determine their own optimal working dilution for each specific research application.

    References
    1. Young, S. et al. (1988) Eur. J. Biochem. 172:247
    2. Young, S. et al. (1987) Biochem. J. 244:775
    Related Products
    Anti-Bovine PKC Protein Kinase C Purified (Leinco Prod. No.: P107)
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    Anti-Bovine PKC-Fluorescein (FITC) (Leinco Prod. No.: P109)

    Products are for research use only. Not for use in diagnostic or therapeutic procedures