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Anti-Human Caspase-14(CT)

Anti-Human Caspase-14(CT)

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Rabbit Anti-Human Caspase-14Purified No Carrier Protein(Mini-interleukin-1 converting enzyme, Mini-ICE, MICE)Polyclonal AntibodyProd. No.: C1249 Host: Rabbit Conc.: 0.5 mg/ml Pkg. Size: 1 mg, 0.1 mg, 0.5 mg Storage: 2-8°C

Rabbit Anti-Human Caspase-14
Purified No Carrier Protein
(Mini-interleukin-1 converting enzyme, Mini-ICE, MICE)
Polyclonal Antibody

Prod. No.: C1249
Host:         Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:       2-8°C

Description
Specificity:
Anti-Human Caspase-14(CT)
Rabbit Anti-Human Caspase-14 recognizes an epitope near the C-terminus of mouse, rat and human Caspase-14. This polyclonal antibody was purified using affinity chromatography.

Background:

Caspases are a family of cysteine proteases that can be divided into apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-14 is highly expressed in embryonic but not adult tissues. It is processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in the keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

Known Species Cross Reactivity: Ms+, Hu+, Rt+

Immunogen
Caspase-14 (CT) Blocking Peptide (Leinco Prod. No.: C1287)
Blocking Peptide
Caspase-14 (CT) Blocking Peptide (Leinco Prod. No.: C1287)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect mouse, rat and human Caspase-14 by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).

References
1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.

2. Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.

3. Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.

4. Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Related Products
Caspase-14 (CT) Blocking Peptide (Leinco Prod. No.: C1287)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.