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Anti-Human Caspase-12(large cleavage product)

Anti-Human Caspase-12(large cleavage product)

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Rabbit Anti-Human Caspase-12Purified No Carrier ProteinPolyclonal AntibodyProd. No.: C1247 Host: Rabbit Conc.: 0.5 mg/ml Pkg. Size: 1 mg, 0.1 mg, 0.5 mg Storage: 2-8°C

Rabbit Anti-Human Caspase-12
Purified No Carrier Protein
Polyclonal Antibody

Prod. No.: C1247
Host:         Rabbit
Conc.:     0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human Caspase-12 recognizes an epitope within the large cleavage product of mouse, rat and human caspase-12. This polyclonal antibody was purified using affinity chromatography.

Background:
Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved.1,2 Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including secretase presenilin and β-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-β. Caspase-12 is ubiquitously expressed in mouse tissues.1

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Caspase-12 (large) Blocking Peptide (Leinco Prod. No.: C1283)
Blocking Peptide
Caspase-12 (large) Blocking Peptide (Leinco Prod. No.: C1283)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect mouse, rat and human Caspase-12 propeptide and cleaved forms by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Heart Tissue Lysate (Leinco Prod. No.: H1405).

References
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000;403:98-103.

2. Mehmet H. Caspases find a new place to hide. Nature 2000;403:29-30

3. Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W. Characterization of seven murine caspase family members. FEBS Lett 1997;403:61-9
Related Products
Caspase-12 (large) Blocking Peptide (Leinco Prod. No.: C1283)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Heart Tissue Lysate (Leinco Prod. No.: H1405)
Caspase-12 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1132)
Caspase-12 (NT) Blocking Peptide (Leinco Prod. No.: C1128)
Anti-Human Caspase-12 (Intermediate Domain) (Leinco Prod. No.: C1115)
Anti-Human Caspase-12 (NT) (Leinco Prod. No.: C1116)
Anti-Human Caspase-12 (small cleavage product) (Leinco Prod. No.: C1248)
Caspase-12 (small) Blocking Peptide (Leinco Prod. No.: C1285)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.