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Anti-Human Caspase-9(Intermediate Domain-1)

Anti-Human Caspase-9(Intermediate Domain-1)

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Rabbit Anti-Human Caspase-9Purified No Carrier Protein(Mch6, Apaf-3)Polyclonal AntibodyProd. No.: C1112 Host: Rabbit Conc.: 0.5 mg/ml Pkg. Size: 1 mg, 0.1 mg, 0.5 mg Storage: 2-8°C

Rabbit Anti-Human Caspase-9
Purified No Carrier Protein
(Mch6, Apaf-3)
Polyclonal Antibody

Prod. No.: C1112
Host:         Rabbit
Conc.:       0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:     2-8°C

Description
Specificity:
Rabbit Anti-Human Caspase-9 recognizes Human Caspase-9. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3.1-3 Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation3 Caspase-9 is also activated by granzyme B and CPP32.1,2 Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis.3 Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD.4 Caspase-9 is expressed in a variety of human tissues.1,2

Known Species Cross Reactivity: Hu+
Immunogen
Caspase-9 (Intermediate Domain-1) Blocking Peptide (Leinco Prod. No.: C1130)
Blocking Peptide
Caspase-9 (Intermediate Domain-1) Blocking Peptide (Leinco Prod. No.: C1130)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human Caspase-9 by Western blot analysis at a concentration of 1:1,000-1:2,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).

References
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89

4. Pan G, ORourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J Biol Chem 1998;273:5841-5 (RD1299)
Related Products
Caspase-9 (Intermediate Domain-1) Blocking Peptide (Leinco Prod. No.: C1130)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
Caspase-9 (Intermediate Domain-2) Blocking Peptide (Leinco Prod. No.: C1131)
Anti-Human Caspase-9 (Intermediate Domain-2) (Leinco Prod. No.: C1113)
Anti-Human Caspase-9 (Leinco Prod. No.: C1458)
Anti-Human Caspase-9 (Antigen Aff. Pur.) (Leinco Prod. No.: C1482)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.