Anti-Human Endostatin(ES)PurifiedMonoclonal AntibodyProd. No.: E131 Clone: NE1.479 Isotype: Mouse IgG1 Conc.: 0.1 mg/ml Pkg. Size: 0.1 mg Storage: -20°C
Prod. No.: E131
Isotype: Mouse IgG1
Conc.: 0.1 mg/ml
Pkg. Size: 0.1 mg
Mouse Anti-Human Endostatin (Clone NE1.479) recognizes Human Endostatin and does not appear to cross-react with Mouse Endostatin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Endostatin is globular domain found at the C-terminal derived from type XVIII collagen. It serves as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It is an angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). 1
On the surface of endothelial cells, endostatin binds with the integrin alpha(5)beta(1) that activates the Src-kinase pathway. The binding of endostatin with integrins also down-regulates the activity of RhoA GTPase and inhibits signaling pathways mediated by small kinases of the Ras and Raf families. All these events promote disassembly of the actin cytoskeleton, disorders in cell-matrix interactions, and decrease in endotheliocyte mobility, i.e., promote the suppression of angiogenesis. 2 Endostatin is currently being studied as part of cancer research.
A peptide corresponding to amino acids 1-12
This purified antibody is formulated in 0.025 M phosphate buffered saline (PBS) pH 7.2 containing 50% glycerol.
Storage and Stability
For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Indirect ELISA, WB
1. Folkman, J. et al. (2006) Exp. Cell. Res . 312: 594
2. Severin SE. et al. (2007) Biochemistry (Mosc). 72: 235
Recombinant Human Endostatin (Leinco Prod. No.: E187)