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Anti-Human MMP-14(MT1-MMP)

Anti-Human MMP-14(MT1-MMP)

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Anti-Human Matrix Metalloproteinase-14(MMP-14)Purified No Carrier Protein(MT1-MMP)Monoclonal AntibodyProd. No.: M1089 Clone: M43.266 Isotype: Mouse IgG1 κ Conc.: 1.0 mg/ml Pkg. Size: 0.2 mg Storage: 2-8°C

Anti-Human Matrix Metalloproteinase-14(MMP-14)
Purified No Carrier Protein
(MT1-MMP)
Monoclonal Antibody
Prod. No.:   M1089
Clone:       M43.266
Isotype:     Mouse IgG1 κ
Conc.:       1.0 mg/ml
Pkg. Size:   0.2 mg
Storage:     2-8°C

Description
Specificity:
Anti-Human MMP-14 (clone M43.266) was produced in mice using a synthetic peptide corresponding to amino acids 160 to 173 of human MMP-14.

Background:
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the eleven proteins reported to date, ten are normally found as soluble molecules. The eleventh, and newest of these proteins, has been named MT-MMP for membrane bound MMP. MT-MMP contains a C-terminal transmembrane domain which allows it to localize to the cell surface. It is also unique from the other members of the MMP family in that it contains an 8 amino acid insert in the catalytic domain. The protein is encoded by a 4.5 kb mRNA species giving rise to a protein with a molecular weight of 60-66 kDa by SDS-PAGE. MT-MMP is responsible for cleaving gelatinase A (MMP-2, 72 kDa Type IV collagenase) to the active form. Evidence exists to suggest that MT-MMP itself requires an activation step which is most likely the result of activity of the membrane plasmin cascade. MT-MMP functions by binding TIMP-2 and then the COOH terminal end of MMP-2 resulting in a 105 kDa trimer which effects the cleavage of pro-MMP-2 to the biologically active form. The order of the binding of pro-MMP-2 and TIMP-2 to MT-MMP is critical as TIMP-2 will also inhibit the activity of MMP-2 when present in a soluble form. Since its discovery, a second form of MT-MMP has been identified resulting in a change in the name of the original protein to MT1-MMP.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
A synthetic peptide corresponding to amino acids 160 to 173 of human MMP-14
Formulation
This purified antibody is formulated with no carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Storage and Stability
The antibody should be stored at -20°C. Avoid repeated freezing and thawing. This product does not contain preservatives.
Reported Applications
WB
Western Blotting: To detect Human MMP-14 by Western blot analysis, this antibody can be used at a concentration of 1 µg/ml. When used in conjunction with compatible second step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). In general, use higher concentrations to enhance sensitivity and lower concentrations to enhance specificity.

References
1. Strongin, A. Y. et al. (1995) J. Biol. Chem. 270:5331
2. Takino, T. et al. (1995) J. Biol. Chem. 270:23013
3. Sato, H. et al. (1994) Nature 370:61
4. Cottam, D. W. et al. (1993) Intl. J. Oncol. 2:861
5. Stetler-Stevenson, W. G. et al. (1993) FASEB J. 7:1434
6. Fridman, R. et al. (1992) J. Biol. Chem. 267:15389
7. Woessner, J. F. (1991) FASEB J. 5:2145
8. Liotta, L. A. et al. (1990) Sem. Cancer Biol. Ed. .M. Gottesman, Vol. 1:99
9. Goldberg, G. I. et al. (1989) Proc. Natl. Acad. Sci. 86:8207

Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)

Products are for research use only. Not for use in diagnostic or therapeutic procedures