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 Carbonic Anhydrase 2/重组大肠杆菌碳酸酐酶2
  • Carbonic Anhydrase 2/重组大肠杆菌碳酸酐酶2
  • Carbonic Anhydrase 2/重组大肠杆菌碳酸酐酶2

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    上架时间:2013年05月08日
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    Carbonic Anhydrase II E.coli重组大肠杆菌碳酸酐酶2Carbonic Anhydrase II E.coli Recombinant Catalog No. ENZ-373
     

    Carbonic Anhydrase II E.coli
    Carbonic Anhydrase II E.coli Recombinant

    Catalog No. ENZ-373

    Synonyms:
    Carbonic anhydrase 2, Carbonate dehydratase 2, can, cynT2, yadF, b0126, JW0122, Carbonic Anhydrase II.

    Introduction:
    The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid and protons (CO2 + H2O ↔ HCO3 + H+), a reaction that occurs rather slowly in the absence of a catalyst. The majority of carbonic anhydrases enclose a zinc ion in their active site and therefore is classified as metalloenzymes.
    The most important function of Carbonic anhydrase is known to preserve acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Carbonic anhydrase has 3 different classes: alpha, beta and gamma which share very little sequence or structural similarity, thus far they all perform the same function and require a zinc ion at the active site. Mammalian carbonic anhydrase is monomeric and belongs to the alpha class. Plant carbonic anhydrase is dimeric and belongs to the beta class.
    Methane-producing bacteria carbonic anhydrase is trimeric and grows in hot springs which forms the gamma class. 

    Description:
    Carbonic anhydrase II is an E.coli Recombinant protein produced in E.Coli containing 240 amino acids (1-220) and having a molecular mass of 27 kDa. Carbonic anhydrase is expressedwith an amino-terminal hexahistidine tag.
    The Carbonic anhydrase 2 is purified by proprietary chromatographic techniques. 

    Source:
    Escherichia Coli.

    Physical Appearance:
    Sterile Filtered colorless solution.

    Formulation:
    The Carbonic Anhydrase 2 enzyme is supplied in 20mM Tris pH-8 and 1mM DTT.

    Stability:
    Carbonic Anhydrase II although stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    Amino Acid Sequence:
    MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLAQAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL HIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK 

    Purity:
    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    Usage:
    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.