Carbonic Anhydrase II E.coli重组大肠杆菌碳酸酐酶2Carbonic Anhydrase II E.coli Recombinant Catalog No. ENZ-373
Carbonic Anhydrase II E.coli
Carbonic Anhydrase II E.coli Recombinant
|Catalog No. ENZ-373 |
Carbonic anhydrase 2, Carbonate dehydratase 2, can, cynT2, yadF, b0126, JW0122, Carbonic Anhydrase II.
The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid ａnd protons （CO2 + H2O ↔ HCO3− + H+）, a reaction that occurs rather slowly in the absence of a catalyst. The majority of carbonic anhydrases enclose a zinc ion in their active site ａnd therefore is classified as metalloenzymes.
The most important function of Carbonic anhydrase is known to preserve acid-base balance in blood ａnd other tissues, ａnd to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Carbonic anhydrase has 3 different classes: alpha, beta ａnd gamma which share very little sequence or structural similarity, thus far they all perform the same function ａnd require a zinc ion at the active site. Mammalian carbonic anhydrase is monomeric ａnd belongs to the alpha class. Plant carbonic anhydrase is dimeric ａnd belongs to the beta class.
Methane-producing bacteria carbonic anhydrase is trimeric ａnd grows in hot springs which forms the gamma class.
Carbonic anhydrase II is an E.coli Recombinant protein produced in E.Coli containing 240 amino acids （1-220） ａnd having a molecular mass of 27 kDa. Carbonic anhydrase is expressedwith an amino-terminal hexahistidine tag.
The Carbonic anhydrase 2 is purified by proprietary chromatographic techniques.
Sterile Filtered colorless solution.
The Carbonic Anhydrase 2 enzyme is supplied in 20mM Tris pH-8 ａnd 1mM DTT.
Carbonic Anhydrase II although stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLAQAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL HIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
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