RC00929B 人单纯疱疹病毒抗原-1(HSV-Ag1)ELISA 试剂盒 人单纯疱疹病毒抗原-1(HSV-Ag1)ELISA Kit
RC00930B 人沙眼衣原体抗体(CT)ELISA 试剂盒 人沙眼衣原体抗体(CT)ELISA Kit
RC00931B 人凝血因子Ⅻ(FⅫ)ELISA 试剂盒 人凝血因子Ⅻ(FⅫ)ELISA Kit
RC00932B 人凝血因子ⅩⅢ(FⅩⅢ)ELISA 试剂盒 人凝血因子ⅩⅢ(FⅩⅢ)ELISA Kit
RC00933B 人凝血因子Ⅲ(FⅢ)ELISA 试剂盒 人凝血因子Ⅲ(FⅢ)ELISA Kit
RC00934B 人血小板因子3(PF-3)ELISA 试剂盒 人血小板因子3(PF-3)ELISA Kit
RC00935B 人凝血酶原片段F1+2(F1+2)ELISA 试剂盒 人凝血酶原片段F1+2(F1+2)ELISA Kit
RC00936B 人血小板相关免疫球蛋白(PAIg)ELISA 试剂盒 人血小板相关免疫球蛋白(PAIg)ELISA Kit
RC00937B 人血小板相关补体3(PAC3)ELISA 试剂盒 人血小板相关补体3(PAC3)ELISA Kit
RC00938B 人血栓素A2(TX-A2)ELISA 试剂盒 人血栓素A2(TX-A2)ELISA Kit
RC00939B 人血管活性肽酶抑制剂(VPI)ELISA 试剂盒 人血管活性肽酶抑制剂(VPI)ELISA Kit
RC00940B 大鼠抗血小板抗体IgG/M/A(PA-IgG/M/A)ELISA 试剂盒 大鼠抗血小板抗体IgG/M/A(PA-IgG/M/A)ELISA Kit
RC00941B 人血红蛋白晚期糖基化终末产物(Hb-AGE)ELISA 试剂盒 人血红蛋白晚期糖基化终末产物(Hb-AGE)ELISA Kit
RC00942B 人血红蛋白β亚基(HB-β)ELISA 试剂盒 人血红蛋白β亚基(HB-β)ELISA Kit
RC00943B 人活化凝血因子Ⅻ(FⅫa)ELISA 试剂盒 人活化凝血因子Ⅻ(FⅫa)ELISA Kit
RC00944B 人异常凝血酶原(APT)ELISA 试剂盒 人异常凝血酶原(APT)ELISA Kit
RC00945B 人凝血酶血栓调节蛋白复合物(T-TM)ELISA 试剂盒 人凝血酶血栓调节蛋白复合物(T-TM)ELISA Kit
RC00946B 人溶血补体(Hc)ELISA 试剂盒 人溶血补体(Hc)ELISA Kit
RC00947B 人2,3Dinor血栓烷B2(2,3-dinor-TXB2)ELISA 试剂盒 人2,3Dinor血栓烷B2(2,3-dinor-TXB2)ELISA Kit
RC00948B 人11去氢血栓烷B2(11-DH-TXB2)ELISA 试剂盒 人11去氢血栓烷B2(11-DH-TXB2)ELISA Kit
Before use, mix all reagents thoroughly without making foam.
Determine the number of microwell strips required to test the desired number of samples,plus appropriate number of wells needed for running blanks standards. Each sample, standard and blank should be assayed in duplicate. Remove sufficient microwell strips from the pouch.
Add 50ul of standard diluent to standard wells B1,B2, C1,C2, D1,D2, E1,E2, F1,F2. Reconstitute standard vial with the appropriate volume as described in the chapter reagents preparation. Preparation. Pipet 100ul of standard into wells A1 and A2 (see plate scheme below). Transfer 50ul from A1 and A2 to B1 and B2 wells. Mix the contents by repeated aspirations and ejections. Take care not to scratch the inner surface of microwells. Repat this procedure from the wells B1,B2 to wells C1,C2 and from wells C1,C2 to D1,D2 and so on creating two parallel rows of α2-MG standard dilutions ranging，Add 50ul of standard diluent to the bland wells.
Dilute samples 1:1 distribing 50ul of sample into 50ul of dilluent ,Add 50ul of diluted sample to wells.
Add 50ul of diluted biotinylated anti-α2-MG to all wells.
Cover with a plate vover and incubate for 1 hour at 37℃.
Remove the cover and wash the plate as follows: ⑴ aspirate the liquid from each well, ⑵ dispensse 0.3ml of washing solution into each well. ⑶ Aspirate again the contet of each well after 0.5 minute. ⑷ Repeat steps ⑵ and ⑶ three times.
Distribute 80ul of streptavidin-HRP solution to all wells, including blank wells.
Cover and incubate 30 min at 37℃.
Remove the cover and empty wells, Wash microwell strips according to step, Proceed immediately to the next step.
Add 50ul Substrate A and Substrate B to each well。Incubate for 10 min at 37℃。
The enzyme-substrate reaction is stopped by quickly pipetting 50ul of H2SO4. stop reagent into each well, including the blank wells, to completely and uniformly inactivate the enzyme. Results must be red immediately after the addition of H2SO4.
Read absorbance of each well on a spectrophotometer using 450nm as the primary wavelength and optionally 620nm (610nm to 650nm is acceptable ) as the reference wavelength.