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 人内脂素/内脏脂肪素(visfatin)ELISA Kit
  • 人内脂素/内脏脂肪素(visfatin)ELISA Kit
  • 人内脂素/内脏脂肪素(visfatin)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC00986B (人内脂素/内脏脂肪素(visfatin)ELISA Kit)
    所在地区:上海
    上架时间:2012年08月01日
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    详细说明

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    SPECIMEN COLLECTION\ PROCESSING AND STORAGE
     Serum---Avoid any inintentional stimulation of the cells by the procedure. Use pyrogen\endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000×g for 10 min and remove serum.
     Plasma---EDTA\ citrate and heparin plasma can be assayed. Spin samples at 1000×g for 30 min remove particulates. Harvest plasma.
     Cell culture supernatants---Remove particulates and aggregates by spinning at approximately 1000×g for 10 min.
     Storage---If not analyzed shortly after collection, samples should be aliquoted(250-500ul) to avoid freeze-thaw cycles and stored frozen at -70℃. Avoid multiple freeze-thaw cycles of frozen specimens. When possible, avoid use of badly hemolyzed or lipemic sera. If large amounts of particles are present, this should be removed prior to assay by centrifugation or filtration.
     Recommendation---Do not thaw by heating at 37℃ or 56℃. Thaw at room temperature and make sure that sample is completely thawed and homogenous before assaying.

    PREPARATION OF REAGENTS
     Standards: Standard have to be reconstituled with the volume of standard buffer diluent indicated on the vial. This reconstitution produces a stock solution of 800ng/ml α2-MG. Allow standard to stand for 5
     minutes with gentle swirling prior to making dilutions. Serial dilutions of standard must be made before each assys and cannot be stored.

    800 ng/ml (6  Standard) Original density 50ul。
    400 ng/ml (5  Standard) 100ul  6 Standard  +100ul diludent
    200 ng/ml (4  Standard) 100ul  5 Standard  +100ul diludent
    100 ng/ml (3  Standard) 100ul  4 Standard  +100ul diludent
    50ng/ml (2  Standard) 100ul  3 Standard  +100ul diludent
    25 ng/ml (1  Standard) 100ul  2 Standard  +100ul diludent
    0 ng/ml Blank Control 50ul。

     Washing buffer 50×concentrate:  Dilute 50 times in distilled water.