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 人抗胸腺细胞球蛋白(ATG)ELISA Kit
  • 人抗胸腺细胞球蛋白(ATG)ELISA Kit
  • 人抗胸腺细胞球蛋白(ATG)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC01129B (人抗胸腺细胞球蛋白(ATG)ELISA Kit )
    所在地区:上海
    上架时间:2012年08月01日
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    详细说明

    RC01129B 人抗胸腺细胞球蛋白(ATG)ELISA 试剂盒  人抗胸腺细胞球蛋白(ATG)ELISA Kit
    RC01130B 人抗IgA抗体(anti-IgA-Ab)ELISA 试剂盒  人抗IgA抗体(anti-IgA-Ab)ELISA Kit
    RC01131B 人甲状腺结合球蛋白(TBG)ELISA 试剂盒  人甲状腺结合球蛋白(TBG)ELISA Kit
    RC01132B 人红细胞膜蛋白(EMP)ELISA 试剂盒  人红细胞膜蛋白(EMP)ELISA Kit
    RC01133B 人高铁血红蛋白(MHB)ELISA 试剂盒  人高铁血红蛋白(MHB)ELISA Kit
    RC01134B 人泛素蛋白(Ub)ELISA 试剂盒  人泛素蛋白(Ub)ELISA Kit
    RC01135B 人TH糖蛋白(THP)ELISA 试剂盒  人TH糖蛋白(THP)ELISA Kit
    RC01136B 人视黄醇结合蛋白4(RBP-4)ELISA 试剂盒 人视黄醇结合蛋白4(RBP-4)ELISA Kit
    RC01137B 人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA 试剂盒 人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA Kit
    RC01138B 人类白细胞抗原G(HLA-G)ELISA 试剂盒 人类白细胞抗原G(HLA-G)ELISA Kit
    RC01139B 人乳腺癌易感蛋白2(BRCA-2)ELISA 试剂盒 人乳腺癌易感蛋白2(BRCA-2)ELISA Kit
    RC01140B 人血凝素(HA)ELISA 试剂盒 人血凝素(HA)ELISA Kit
    RC01141B 人脂肪分化相关蛋白(ADRP)ELISA 试剂盒 人脂肪分化相关蛋白(ADRP)ELISA Kit
    RC01142B 人垂体腺苷酸环化酶激活肽(PACAP)ELISA 试剂盒 人垂体腺苷酸环化酶激活肽(PACAP)ELISA Kit
    RC01143B 人Bcl-2相关X蛋白(BAX)ELISA 试剂盒  人Bcl-2相关X蛋白(BAX)ELISA Kit
    RC01144B 人α平滑肌肌动蛋白(α-SMA)ELISA 试剂盒  人α平滑肌肌动蛋白(α-SMA)ELISA Kit
    RC01145B 人α横纹肌肌动蛋白(α-SCA)ELISA 试剂盒 人α横纹肌肌动蛋白(α-SCA)ELISA Kit
    RC01146B 人平滑肌肌球蛋白(SMM)ELISA 试剂盒  人平滑肌肌球蛋白(SMM)ELISA Kit
    RC01147B 人心肌特异性肌钙蛋白T(cTnT)ELISA 试剂盒  人心肌特异性肌钙蛋白T(cTnT)ELISA Kit
    RC01148B 人肌球蛋白重链(MHC)ELISA 试剂盒  人肌球蛋白重链(MHC)ELISA Kit
    RC01149B 人弹性蛋白(Elastin)ELISA 试剂盒  人弹性蛋白(Elastin)ELISA Kit
    RC01150B 人套膜蛋白(iNV)ELISA 试剂盒 人套膜蛋白(iNV)ELISA Kit
    RC01151B 人微管相关蛋白2(MAP-2)ELISA 试剂盒 人微管相关蛋白2(MAP-2)ELISA Kit
    ASSAY METHOD
     Before use, mix all reagents thoroughly without making foam.
     Determine the number of microwell strips required to test the desired number of samples,plus appropriate number of wells needed for running blanks standards. Each sample, standard and blank should be assayed in duplicate. Remove sufficient microwell strips from the pouch.
     Add 50ul of standard diluent to standard wells B1,B2,  C1,C2,  D1,D2,  E1, E2,  F1,F2. Reconstitute standard vial with the appropriate volume as described in the chapter reagents preparation. Preparation. Pipet 100ul of standard into wells A1 and A2 (see plate scheme below). Transfer 50ul from A1 and A2 to B1 and B2 wells. Mix the contents by repeated aspirations and ejections. Take care not to scratch the inner surface of microwells. Repat this procedure from the wells B1,B2 to wells C1,C2 and from wells C1,C2 to D1,D2 and so on creating two parallel rows of GMP-140 standard dilutions ranging,Add 50ul of standard diluent to the bland wells.
     Dilute samples 1:1 distribing 50ul of sample into 50ul of dilluent ,Add 50ul of diluted sample to wells..
     Add 50ul of diluted biotinylated anti-GMP-140 to all wells.
     Cover with a plate vover and incubate for 1 hour at 37℃.
     Remove the cover and wash the plate as follows: ⑴ aspirate the liquid from each well, ⑵ dispensse 0.3ml of washing solution into each well. ⑶ Aspirate again the contet of each well after 0.5 minute. ⑷ Repeat steps ⑵ and ⑶ three times.
     Distribute 80ul of streptavidin-HRP solution to all wells, including blank wells.
     Cover and incubate 30 min at 37℃.
     Remove the cover and empty wells, Wash microwell strips according to step, Proceed immediately to the next step.
     Add 50ul Substrate A and Substrate B to each well。Incubate for 10 min at 37℃。
     The enzyme-substrate reaction is stopped by quickly pipetting 50ul of H2SO4. stop reagent into each well, including the blank wells, to completely and uniformly inactivate the enzyme. Results must be red immediately after the addition of H2SO4.
     Read absorbance of each well on a spectrophotometer using 450nm as the primary wavelength and optionally 620nm (610nm to 650nm is acceptable ) as the reference wavelength.