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 人α烯醇化酶(α-enolase)ELISA Kit
  • 人α烯醇化酶(α-enolase)ELISA Kit
  • 人α烯醇化酶(α-enolase)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC01337B (人α烯醇化酶(α-enolase)ELISA Kit )
    所在地区:上海
    上架时间:2012年08月01日
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    SPECIMEN COLLECTION\ PROCESSING AND STORAGE
     Serum---Avoid any inintentional stimulation of the cells by the procedure. Use pyrogen\endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000×g for 10 min and remove serum.
     Plasma---EDTA\ citrate and heparin plasma can be assayed. Spin samples at 1000×g for 30 min remove particulates. Harvest plasma.
     Cell culture supernatants---Remove particulates and aggregates by spinning at approximately 1000×g for 10 min.
     Storage---If not analyzed shortly after collection, samples should be aliquoted(250-500ul) to avoid freeze-thaw cycles and stored frozen at -70℃. Avoid multiple freeze-thaw cycles of frozen specimens. When possible, avoid use of badly hemolyzed or lipemic sera. If large amounts of particles are present, this should be removed prior to assay by centrifugation or filtration.
     Recommendation---Do not thaw by heating at 37℃ or 56℃. Thaw at room temperature and make sure that sample is completely thawed and homogenous before assaying.

    PREPARATION OF REAGENTS
     Standards: Standard have to be reconstituled with the volume of standard buffer diluent indicated on the vial. This reconstitution produces a stock solution of 40IU/ml AMCA. Allow standard to stand for 5
     minutes with gentle swirling prior to making dilutions. Serial dilutions of standard must be made before each assys and cannot be stored.

    40 IU/ml (6  Standard) Original density 50ul。
    20 IU/ml (5  Standard) 100ul  6 Standard  +100ul diludent
    10 IU/ml (4  Standard) 100ul  5 Standard  +100ul diludent
    5.0 IU/ml (3  Standard) 100ul  4 Standard  +100ul diludent
    2.5IU/ml (2  Standard) 100ul  3 Standard  +100ul diludent
    1.25 IU/ml (1  Standard) 100ul  2 Standard  +100ul diludent
    0 IU/ml Blank Control 50ul。

     Washing buffer 50×concentrate:  Dilute 50 times in distilled water.

    样品收集、处理及保存方法
    1、 血清-----操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清和红细胞迅速小心地分离。
    2、 血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
    3、 细胞上清液---1000×g离心10分钟去除颗粒和聚合物。
    4、 组织匀浆-----将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液
    5、 保存------如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。