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 大鼠己糖激酶(HK)ELISA Kit
  • 大鼠己糖激酶(HK)ELISA Kit
  • 大鼠己糖激酶(HK)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC01838B (大鼠己糖激酶(HK)ELISA Kit)
    所在地区:上海
    上架时间:2012年08月01日
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    SAFETY
     For research use only
     Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly water.
     Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
     Do not pipette by mouth.

    PROCEDURAL NOTES/LAB.QUALITY CONTROL
     When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.
     Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from edterioration.
     Cover or cap all reagents when not in use.
     Do not mis or interchange reagents between different lots.
     Do not use reagents beyond the expiration date of the kit .
     Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 and substrate solution, avoid pipettes with metal parts.
     Use a clean plastic container to prepare the washing solution.
     Thoroughly mix the reagents and samples before use by agitation or swirling.
     All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
     The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated and must be discarede. Read absorbances within 1 hour after completion of the assay.
     When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.
     Respect incubation times described in the assay procedure.

    SPECIMEN COLLECTION\ PROCESSING AND STORAGE
     Serum---Avoid any inintentional stimulation of the cells by the procedure. Use pyrogen\endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000×g for 10 min and remove serum.
     Plasma---EDTA\ citrate and heparin plasma can be assayed. Spin samples at 1000×g for 30 min remove particulates. Harvest plasma.
     Cell culture supernatants---Remove particulates and aggregates by spinning at approximately 1000×g for 10 min.
     Storage---If not analyzed shortly after collection, samples should be aliquoted(250-500ul) to avoid freeze-thaw cycles and stored frozen at -70℃. Avoid multiple freeze-thaw cycles of frozen specimens. When possible, avoid use of badly hemolyzed or lipemic sera. If large amounts of particles are present, this should be removed prior to assay by centrifugation or filtration.
     Recommendation---Do not thaw by heating at 37℃ or 56℃. Thaw at room temperature and make sure that sample is completely thawed and homogenous before assaying.