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 大鼠糖原合成酶激酶(GSK)ELISA Kit
  • 大鼠糖原合成酶激酶(GSK)ELISA Kit
  • 大鼠糖原合成酶激酶(GSK)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC01839B (大鼠糖原合成酶激酶(GSK)ELISA Kit)
    所在地区:上海
    上架时间:2012年08月01日
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    PRINCIPLE OF THE METHOD
    The β2-MG kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of  known β2-MG concentrations and unknowns are pipetted into these wells. During the first incubation, the β2-MG antigen and a biotinylated monoclonal antibody specific for β2-MG are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of β2-MG present in the samples.

    REAGENTS PROVIDED AND RECONSTTTUTION
    REAGENTS(Store at 2-8℃) 1×96 WELLS 0.5×96 WELLS RECONSTTTUTION
    96/48-wells microtiter plates 1 0.5 Ready-to-use
    Plastiv cover 2 1 Ready-to-use
    Standard: 800ng/ml 1Vials  (0.6ml) 0.5Vials  (0.3ml) See reagents preparation on page 3
    Blank control 1Vials  (1.0ml) 1Vials   (0.5ml) Ready-to-use
    Standard Diluent 1Vials  (5ml) 1Vials   (2.5ml) Ready-to-use
    Biotinylated anti-β2-MG 1Vials  (6ml) 1Vials   (3.0ml) Ready-to-use
    Streptavidin-HRP 1Vials  (10ml) 1Vials   (5.0ml) Ready-to-use
    Washing Buffer 1Vials  (20ml) 1Vials   (10ml) 50× concentrate
    Substrate  A 1Vials  (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Substrate  B 1Vials (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Stopping Solution 1Vials  (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Sample Diluent  1Vials  (12ml) 1Vials   (6.0ml) Ready-to-use


    MATERIAL REQUIRED BUT NOT PROVIDED
     Distilled water
     Pipettes:10ul、50ul、100ul、200ul、1000ul。
     Vortex mixer and magnetic stirrer.

    SAFETY
     For research use only
     Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly water.
     Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
     Do not pipette by mouth.

    PROCEDURAL NOTES/LAB.QUALITY CONTROL
     When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.
     Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from edterioration.
     Cover or cap all reagents when not in use.
     Do not mis or interchange reagents between different lots.
     Do not use reagents beyond the expiration date of the kit .
     Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 and substrate solution, avoid pipettes with metal parts.
     Use a clean plastic container to prepare the washing solution.
     Thoroughly mix the reagents and samples before use by agitation or swirling.
     All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
     The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated and must be discarede. Read absorbances within 1 hour after completion of the assay.
     When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.
     Respect incubation times described in the assay procedure.