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 猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit
  • 猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit
  • 猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit

    参考价格: ¥3000.00
    品  牌:USA
    产品型号:RC02211B (猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit)
    所在地区:上海
    上架时间:2012年08月01日
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    RC02211B 猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA 试剂盒 猴子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit
    RC02212B 猴子神经胶质纤维酸性蛋白(GFAP)ELISA 试剂盒 猴子神经胶质纤维酸性蛋白(GFAP)ELISA Kit
    RC02213B 鸭主要组织相容性复合体(MHC)ELISA 试剂盒  鸭主要组织相容性复合体(MHC)ELISA Kit
    RC02214B 鸭病毒性肠炎病毒(DEV)ELISA 试剂盒 鸭病毒性肠炎病毒(DEV)ELISA Kit
    RC02215B 鸭白介素6(IL-6)ELISA 试剂盒  鸭白介素6(IL-6)ELISA Kit
    RC02216B 鸭白介素1(IL-1)ELISA 试剂盒  鸭白介素1(IL-1)ELISA Kit
    RC02217B 鸭γ干扰素(IFN-γ)ELISA 试剂盒 鸭γ干扰素(IFN-γ)ELISA Kit
    RC02218B 鸭白介素8(IL-8/CXCL8)ELISA 试剂盒 鸭白介素8(IL-8/CXCL8)ELISA Kit
    RC02219B 鸭白介素4(IL-4)ELISA 试剂盒 鸭白介素4(IL-4)ELISA Kit
    RC02220B 鸭白介素2(IL-2)ELISA 试剂盒 鸭白介素2(IL-2)ELISA Kit
    RC02221B 鸭子碳酸酐酶(CA)ELISA 试剂盒 鸭子碳酸酐酶(CA)ELISA Kit
    RC02222B 鸭抗凝血素抗体(aPT1/aPT2)ELISA 试剂盒 鸭抗凝血素抗体(aPT1/aPT2)ELISA Kit
    RC02223B 鱼类主要组织相容性复合体(MHC)ELISA 试剂盒 鱼类主要组织相容性复合体(MHC)ELISA Kit
    RC02224B 鲑鱼补体蛋白3(C3)ELISA 试剂盒 鲑鱼补体蛋白3(C3)ELISA Kit
    RC02225B 鲑鱼补体蛋白4(C4)ELISA 试剂盒  鲑鱼补体蛋白4(C4)ELISA Kit
    RC02226B 鱼类白介素1α(IL-1α)ELISA 试剂盒  鱼类白介素1α(IL-1α)ELISA Kit
    RC02227B 鱼类白介素1β (IL-1β)ELISA 试剂盒 鱼类白介素1β (IL-1β)ELISA Kit
    RC02228B 鲤鱼卵黄蛋白原(VTG)ELISA 试剂盒 鲤鱼卵黄蛋白原(VTG)ELISA Kit
    RC02229B 鱼类促性腺激素释放激素(GnRH)ELISA 试剂盒  鱼类促性腺激素释放激素(GnRH)ELISA Kit
    RC02230B 鲑鱼降钙素(SCT)ELISA 试剂盒 鲑鱼降钙素(SCT)ELISA Kit
    RC02231B 鱼类甲状腺素(T4)ELISA 试剂盒 鱼类甲状腺素(T4)ELISA Kit
    RC02232B 鱼类三碘甲状腺原氨酸(T3)ELISA 试剂盒 鱼类三碘甲状腺原氨酸(T3)ELISA Kit
    RC02233B 鱼类皮质醇(Cortisol)ELISA 试剂盒 鱼类皮质醇(Cortisol)ELISA Kit
    RC02234B 鱼类超敏C反应蛋白(hs-CRP)ELISA 试剂盒 鱼类超敏C反应蛋白(hs-CRP)ELISA Kit
    RC02235B 鱼骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA 试剂盒 鱼骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA Kit
    RC02236B 山羊主要组织相容性复合体(MHC/OLA)ELISA 试剂盒 山羊主要组织相容性复合体(MHC/OLA)ELISA Kit
    RC02237B 山羊白介素2受体(IL-2R)ELISA 试剂盒 山羊白介素2受体(IL-2R)ELISA Kit
    RC02238B 山羊丙酮检测(acetone)ELISA 试剂盒 山羊丙酮检测(acetone)ELISA Kit
    RC02239B 山羊γ干扰素(IFN-γ)ELISA 试剂盒  山羊γ干扰素(IFN-γ)ELISA Kit
    RC02240B 山羊肿瘤坏死因子α(TNF-α)ELISA 试剂盒  山羊肿瘤坏死因子α(TNF-α)ELISA Kit
    RC02241B 山羊白介素2(IL-2)ELISA 试剂盒  山羊白介素2(IL-2)ELISA Kit
    RC02242B 山羊白介素10(IL-10)ELISA 试剂盒 山羊白介素10(IL-10)ELISA Kit
    RC02243B 山羊白介素4(IL-4)ELISA 试剂盒  山羊白介素4(IL-4)ELISA Kit
    REAGENTS PROVIDED AND RECONSTTTUTION
    REAGENTS(Store at 2-8℃) 1×96 WELLS 0.5×96 WELLS RECONSTTTUTION
    96/48-wells microtiter plates 1 0.5 Ready-to-use
    Plastiv cover 2 1 Ready-to-use
    Standard: 1600ng/L 1Vials  (0.6ml) 0.5Vials  (0.3ml) See reagents preparation on page 3
    Blank control 1Vials  (1.0ml) 1Vials   (0.5ml) Ready-to-use
    Standard Diluent 1Vials  (5ml) 1Vials   (2.5ml) Ready-to-use
    Biotinylated anti-Dopamine 1Vials  (6ml) 1Vials   (3.0ml) Ready-to-use
    Streptavidin-HRP 1Vials  (10ml) 1Vials   (5.0ml) Ready-to-use
    Washing Buffer 1Vials  (20ml) 1Vials   (10ml) 50× concentrate
    Substrate  A 1Vials  (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Substrate  B 1Vials (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Stopping Solution 1Vials  (6.0ml) 1Vials   (3.0ml) Ready-to-use
    Sample Diluent  1Vials  (12ml) 1Vials   (6.0ml) Ready-to-use


    MATERIAL REQUIRED BUT NOT PROVIDED
     Distilled water
     Pipettes:10ul、50ul、100ul、200ul、1000ul。
     Vortex mixer and magnetic stirrer.

    SAFETY
     For research use only
     Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly water.
     Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
     Do not pipette by mouth.

    PROCEDURAL NOTES/LAB.QUALITY CONTROL
     When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.
     Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from edterioration.
     Cover or cap all reagents when not in use.
     Do not mis or interchange reagents between different lots.
     Do not use reagents beyond the expiration date of the kit .
     Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 and substrate solution, avoid pipettes with metal parts.
     Use a clean plastic container to prepare the washing solution.
     Thoroughly mix the reagents and samples before use by agitation or swirling.
     All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
     The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated and must be discarede. Read absorbances within 1 hour after completion of the assay.
     When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.
     Respect incubation times described in the assay procedure.

    SPECIMEN COLLECTION\ PROCESSING AND STORAGE
     Serum---Avoid any inintentional stimulation of the cells by the procedure. Use pyrogen\endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000×g for 10 min and remove serum.
     Plasma---EDTA\ citrate and heparin plasma can be assayed. Spin samples at 1000×g for 30 min remove particulates. Harvest plasma.
     Cell culture supernatants---Remove particulates and aggregates by spinning at approximately 1000×g for 10 min.
     Storage---If not analyzed shortly after collection, samples should be aliquoted(250-500ul) to avoid freeze-thaw cycles and stored frozen at -70℃. Avoid multiple freeze-thaw cycles of frozen specimens. When possible, avoid use of badly hemolyzed or lipemic sera. If large amounts of particles are present, this should be removed prior to assay by centrifugation or filtration.
     Recommendation---Do not thaw by heating at 37℃ or 56℃. Thaw at room temperature and make sure that sample is completely thawed and homogenous before assaying.