上海研域商贸有限公司

 PC-3(人前列腺癌细胞)
  • PC-3(人前列腺癌细胞)
  • PC-3(人前列腺癌细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CRL-1435 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    PC-3(人前列腺癌细胞)

    PC-3(人前列腺癌细胞)
     
    Cell Biology
    ATCC® Number: CRL-1435™ Price: $203.00
    Designations:
    PC-3
    Depositors:
    ME Kaighn
    Biosafety Level:
    1
    Shipped:
    frozen
    Medium & Serum:
    See Propagation
    Growth Properties:
    adherent(The cells form clusters in soft agar and can be adapted to suspension growth)
    Organism:
    Homo sapiens (human)
    Morphology:
    epithelial
    PC-3(人前列腺癌细胞)
     
    Source:
    Organ: prostate
    Disease: adenocarcinoma
    Tumor stage: grade IV
    Derived from metastatic site: bone
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    transfection host (technology from amaxa
    Roche FuGENE® Transfection Reagents)
    Tumorigenic:
    Yes, in semi-solid medium
    Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
    Antigen Expression:
    HLA A1, A9
    DNA Profile
    (STR):
    Amelogenin: X
    CSF1PO: 11
    D13S317: 11
    D16S539: 11
    D5S818: 13
    D7S820: 8,11
    THO1: 6,7
    TPOX: 8,9
    vWA: 17
    Cytogenetic
    Analysis:
    The line is near-triploid with a modal number of 62 chromosomes. There are nearly 20 marker chromosomes commonly found in each cell; and normal N2, N3, N4, N5, N12, and N15 are not found. No normal Y chromosomes could be detected by Q-band analysis.
    Age:
    62 years adult
    Gender:
    male
    Ethnicity:
    Caucasian
    Comments:
    The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. [22363]
    The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing:
    Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.


    Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

    Medium renewal: 2 to 3 times per week
    Preservation:
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004
    recommended serum: ATCC 30-2020
    References:
    22363: Kaighn ME , et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482
    22470: Chen TR . Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522
    26302: Ohnuki Y , et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073
    32341: Sheng S , et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194
    32344: Umekita Y , et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218
    32460: Carter RE , et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628
    32486: Nupponen NN , et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501
    32488: Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241
    32916: Su ZZ , et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978