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 Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B](人肝癌细胞)
  • Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B](人肝癌细胞)
  • Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B](人肝癌细胞)

    参考价格: ¥0.00
    品  牌:ATCC
    产品型号:HB-8064 (株)
    所在地区:上海
    上架时间:2012年08月01日
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    Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B](人肝癌细胞)

    Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B](人肝癌细胞)
     
    Cell Biology
    ATCC® Number: HB-8064™ Price: $203.00
    Designations:
    Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B]
    Depositors:
    Wistar Institute
    2 [Cells Contain HEPATITIS B]
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Homo sapiens (human)
    Morphology:
    epithelial
     
    Source:
    Organ: liver
    Disease: hepatocellular carcinoma
    Cellular Products:
    alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; [3525],alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); [3525],alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin [3525]
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims.
    Applications:
    Tumorigenic:
    Yes, forms tumors in nude mice
    DNA Profile
    (STR):
    Amelogenin: X
    CSF1PO: 8
    D13S317: 12,14
    D16S539: 10
    D5S818: 13
    D7S820: 8,10
    THO1: 6,7
    TPOX: 9
    vWA: 17
    Cytogenetic
    Analysis:
    modal number = 60 with a subtetraploid mode of 82; has a rearranged chromosome 1
    Age:
    8 years juvenile
    Gender:
    male
    Ethnicity:
    Black
    Comments:
    This line contains an integrated hepatitis B virus genome. [1205] [3525]
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

    Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

    Medium renewal: Twice per week
    Preservation:
    Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
    recommended serum: ATCC 30-2020
    References:
    1205: Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960
    3525: Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
    22909: Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137
    24388: Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851