Medium & Serum:
Homo sapiens (human)
|Source: || |
Cell type: fibroblast
|Permits/Forms: || |
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|Related Cell Culture Products |
|Isolation: || |
Isolation date: September, 1966
|Applications: || |
efficacy testing 
testing  
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing  
poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)
|Reverse Transcript: || |
|DNA Profile |
Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.
|Isoenzymes: || |
|Age: || |
14 weeks gestation
|Gender: || |
|Ethnicity: || |
|Comments: || |
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966.
The cells are capable of 42 to 46 population doublings before the onset of senescence.
|Propagation: || |
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|Subculturing: || |Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
A subcultivation ratio of 1:2 to 1:5 is recommended Medium renewal:
1 to 2 times per week
|Preservation: || |
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temerpature
|Related Products: || |
Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
recommended serum: ATCC 30-2020
irradiated to be used as feeder cells: ATCC 55-X
|References: || |
22893: Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953
26036: Proc. Symp. Human Diploid Cells. 227: Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55.
26037: Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262
26038: Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218
32288: Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
32696: Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893
92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia: Standards Australia; Standards Australia AS ISO 10993.5-2002.
92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 10993-5:1999.
92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 1838-02.
92582: Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 2011-99.
92587: Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 2197-02.