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  • RAW 264.7(单核巨噬细胞白血病)

    参考价格: ¥0.00
    品  牌:ATCC
    产品型号:TIB-71 (株)
    所在地区:上海
    上架时间:2012年08月01日
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    RAW 264.7(单核巨噬细胞白血病)

    RAW 264.7(单核巨噬细胞白血病)
     
    Cell Biology
    ATCC® Number: TIB-71™ Price: $203.00
    Designations:
    RAW 264.7
    Depositors:
    WC Raschke
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Mus musculus (mouse)
    Morphology:
    monocyte/macrophage
     
    Source:
    Tissue: ascites
    Cell type: macrophage; Abelson murine leukemia virus transformed
    Disease: Abelson murine leukemia virus-induced tumor
    Cellular Products:
    lysozyme [1207]
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    Biological response [92560]
    transfection host (Roche FuGENE® Transfection Reagents
    technology from amaxa)
    Receptors:
    complement (C3) [1207]
    Antigen Expression:
    H-2d
    Strain:
    BALB/c
    Age:
    adult
    Gender:
    male
    Comments:
    This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2) This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. Unpublished data communicated in Feb. 2007 by Dr Janet W. Hartley, Scientist Emerita, NIAID, indicates the expression of infectious ecotropic MuLV closely related, if not identical, to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV, unsurprisingly based on the mouse passage history of the virus stocks.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing:
    Protocol: Subcultures are prepared by scraping.
    For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.

    Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

    Medium renewal: Replace or add medium every 2 to 3 days.
    Preservation:
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
    recommended serum: ATCC 30-2020
    References:
    1135: Ralph P , Nakoinz I . Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
    1207: Raschke WC , et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
    32443: Denlinger LC , et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
    32466: Hambleton J , et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
    32553: Taylor GA , et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
    32901: Li YM , et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
    33046: Panneerselvam K , Freeze HH . Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
    33076: Lokuta MA , et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857
    33162: Taylor MF , et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
    92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA: ASTM International; ASTM Standard Test Method F 1903-98R03.