Synonym Names for CSF3 protein: CSF3; GCSF; G-CSF, Human, Recombinant, E Coli; G-CSF; Pluripoietin; Filgrastim; Lenograstim; Granulocyte colony-stimulating factor
Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells.
In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity.
Recombinant hG-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Subcellular Location: Secreted.
Ptm: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Pharmaceutical: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).
Similarity: Belongs to the IL-6 superfamily.
G-CSF reacts with the proliferative activity of rhg-csf is tested in culture using mouse bone marrow cells..