北京雅安达生物技术有限公司

 Sfi I from Streptomyces fimbriatus1,250 U (10 U/μl)(Roche) 编号:11288024001
  • Sfi I from Streptomyces fimbriatus1,250 U (10 U/μl)(Roche) 编号:11288024001
  • Sfi I from Streptomyces fimbriatus1,250 U (10 U/μl)(Roche) 编号:11288024001

    参考价格: ¥0.00
    所在地区:北京
    上架时间:2013年04月07日
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

     名称:原装供应| Sfi I from Streptomyces fimbriatus
    货号:11288024001
    规格:1,250 U (10 U/µl)
    产地:Roche
    价格:2002
    Source: From Streptomyces fimbriatus 
    Special incubation temperature: 50°C
    Product Description
    Heat inactivation: Sfi I cannot be heat inactivated by 15 min incubation at 65°C.
    Compatible ends: For an  Sfi I end  to be compatible and ligatable with any other end, the two corresponding recognition sites must have contained the same three internal Ns.
    Unit definition: One unit is the enzyme activity that completely cleaves 1 µg Ad2 DNA in 1 h at 50°C in a total volume of 25 µl SuRE/Cut buffer M.
    Application
    Isoschizomers:  The enzyme has no known isoschizomers. Because of its unusual recognition sequence, Sfi I is useful for analyzing and cloning large DNA fragments. Methylation sensitivity: Sfi I is inhibited by the presence of 5-methylcytosine at the most central C residue (*) on the recognition sequence. 5-methylcytosine at the other C-positions (°) will not inhibit the enzyme.