名称：原装现货| TKB1 Competent Cells
规格：5 x 0.2 m
Store cells immediately at –80°C. Do not place the cells in liquid nitrogen. If the target protein is cloned as a fusion to an affinity tag, purification of large amounts of phosphorylated protein is relatively easy.
The TKB1 strain harbors a plasmidencoded, inducible tyrosine kinase gene (pTK). For best results, please follow the directions outlined in the following sections. The TKB1 strain can be transformed with a plasmid (ColE1 origin) containing a DNA sequence encoding a phosphorylation target domain or protein. The phosphorylated protein isolated from the TK strain can be used to screen expression libraries and to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target. It is important to store the competent cells at –80°C to prevent a loss of efficiency.