北京雅安达生物技术有限公司

 TKB1 Competent Cells5 x 0.2 m(Agilent) 编号:200134
  • TKB1 Competent Cells5 x 0.2 m(Agilent) 编号:200134
  • TKB1 Competent Cells5 x 0.2 m(Agilent) 编号:200134

    参考价格: ¥0.00
    所在地区:北京
    上架时间:2013年04月07日
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     名称:原装现货| TKB1 Competent Cells 
    货号:200134
    产地:Agilent
    规格:5 x 0.2 m
    价格:4884
    STORAGE CONDITIONS
    Store cells immediately at –80°C. Do not place the cells in liquid nitrogen. If the target protein is cloned as a fusion to an affinity tag, purification of large amounts of phosphorylated protein is relatively easy.
    INTRODUCTION
    The TKB1 strain harbors a plasmidencoded, inducible tyrosine kinase gene (pTK). For best results, please follow the directions outlined in the following sections. The TKB1 strain can be transformed with a plasmid (ColE1 origin) containing a DNA sequence encoding a phosphorylation target domain or protein. The phosphorylated protein isolated from the TK strain can be used to screen expression libraries and to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target. It is important to store the competent cells at –80°C to prevent a loss of efficiency.