北京雅安达生物技术有限公司

 hCD4+/45RA- T Cell Column 4 PK1 KT(R&D) 编号:HCD42
  • hCD4+/45RA- T Cell Column 4 PK1 KT(R&D) 编号:HCD42
  • hCD4+/45RA- T Cell Column 4 PK1 KT(R&D) 编号:HCD42

    参考价格: ¥0.00
    所在地区:北京
    上架时间:2013年04月07日
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     名称:正品原装| hCD4+/45RA- T Cell Column 4 PK 
    货号:HCD42
    产地:R&D
    规格:1 KT
    价格:5860
    Store all reagents at 2 - 8° C.
    Intended Use
    Designed to isolate sub-populations of CD4+/CD45RA- human T cells via high affinity negative selection. This may be achieved by warming the 10X column buffer bottle in a 37° C water bath for 5 - 10 mintues. The resulting column eluate is a highly enriched T cell subset population with minimal contamination of either CD45RA+ or CD8+ cells. Process cells on a density gradient, like Ficoll Hypaque, to enrich for mononuclear cells.
    Principle of Selection
    Leukocyte suspensions are incubated with a mixture of monoclonal antibodies and then loaded onto T Cell Subset Columns. Should this be the case, do not carry out the 1:10 buffer dilution (as indicated in step #1) until all salts are in solution. B cells and non-selected T cell subsets bind to anti-Ig coated glass beads via F(ab)-surface immunoglobulin (Ig) interactions, while monocytes bind to Ig coated glass beads via Fc interactions.