名称：正品原装| hEndoglin+ PlusCellect
Reagents are stable for 12 months from the date of receipt when stored in the dark at 2 - 8° C. DO NOT FREEZE. Purity of recovered Endoglin+ cells typically ranges between 60 - 85%.
The Human Endoglin/CD105 PlusCellect Kit is designed to isolate cells via a positive selection principle. Use sterile equipment when pipetting reagents that will be reused at a later date. The resulting cell preparation is highly enriched for Endoglin+ cells. If sterile cells are required following the cell selection, the entire procedure should be carried out in a laminar flow hood to maintain sterile conditions.
Fc receptor blocking (step 2 of the Cell Selection Procedure) can be enhanced by also adding 25 uL of autologous plasma per 107 cells being processed. Flush with large volumes of water during disposal. Keep the biotinylated antibody and ferrofluid incubation times the same. The PE-conjugated detection antibody provided in this kit contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Keep the cell density at 1 x 107 cells/mL. If blocking, add 100 g of human IgG per 107 cells being processed.