名称：正品原装| rCD8+ T Cell Column, Sm 4 PK
Store all reagents at 2 - 8° C.
Designed to prepare sub-populations of CD8+ rat T cells via high affinity negative selection. The cells will displace some of the buffer in the column which can be collected. The resulting column eluate contains a highly enriched T cell subset population with minimal presence of B cells, monocytes, or non-selected T cells. The resulting column eluate is a highly enriched T cell subset population of the desired T cells.
Principle of Selection
Mononuclear cell suspensions are incubated with a mixture of monoclonal antibodies and then loaded onto T Cell Subset Columns. The column is washed with 10 mL of 1X Column Buffer and the eluate is also allowed to drain into a waste receptacle. B cells, non-selected T cells and monocytes bind to glass beads coated with anti-immunoglobulin via both F(ab) and Fc interactions. The antibody treated cells are applied to a Subset Column and allowed to enter into the column.