名称：正品订购| hgc/IL-2 Rg PE MAb (31134)
Storage: Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C.
Should this be the case, surface staining of cells prior to fixation and permeabilization is recommended. The reagent is designed for flow cytometric applications intended to identify and quantitate cells possessing cytoplasmic forms of the protein recognized by the monoclonal. Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells.
If it is desirable to stain for surface proteins, such as CD3, CD4, CD8, etc., Cells are initially fixed in order to minimize leakage of proteins out of the cell. in addition to the intracellular staining properties of the cells, we recommend that the investigator determine whether the surface protein is adversely affected by the fixation and permeabilization steps. Fixed cells are permeabilized, allowing conjugated antibodies access to proteins within the cell.