名称：正品订购| Human IL-1 alpha/IL-1F1 DuoSet
It is recommended that all standards and samples be assayed in duplicate. The following factors prepared at 50 ng/mL were assayed and exhibited no cross-reactivity or interference. A basic understanding of immunoassay development is required for the successful use of these reagents in immunoassays. It is important that the diluents selected for reconstitution and for dilution of the standard reflect the environment of the samples being measured. Use a fresh reagent reservoir and pipette tips for each step.
TECHNICAL HINTS AND LIMITATIONS
This DuoSet should not be used beyond the expiration date on the label. The diluent suggested in this protocol should be suitable for most cell culture supernate samples. Validate diluents for specific sample types prior to use. The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing
protection when using this material. The type of enzyme and substrate and the concentrations of capture/detection antibodies used can be varied to create an immunoassay with a different sensitivity and dynamic range.