名称：正品订购| hIL-13 Ra1 APC MAb (Cl 419718)
Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C. Unbound allophycocyanin-conjugated antibody is then washed from the cells.
Designed to quantitatively determine the percentage of cells bearing IL-13 Rα1 within a population and qualitatively determine the density of IL-13 Rα1 on cell surfaces by flow cytometry. Cell surface expression of IL-13 Rα1 is determined by flow cytometry using 620 - 650 nm wavelength laser excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 660 - 670 nm.
Principle of the Test
Washed cells are incubated with the allophycocyanin-labeled monoclonal antibody, which binds to cells expressing IL-13 Rα1. Cells should then be resuspended in the same buffer to a final concentration of 4 x 106 cells/mL and 25 μL of cells (1 x 105) transferred to a 5 mL tube for staining. Cells expressing IL-13 Rα1 are fluorescently stained, with the intensity of staining directly proportional to the density of expression of IL-13 Rα1.