名称：正品订购| hIL-17B Fluor MAb (Cl 174113)
Storage: 2° - 8° C in the dark for up to twelve months without a significant loss of activity.
The reagent is designed for flow cytometric applications intended to identify and quantitate cells expressing cytoplasmic forms of the protein recognized by the monoclonal. Centrifuge the cells at 200 x g for 7 minutes. Cells are initially subjected to a fixation step in order to minimize leakage of proteins out of the cell.
Principle of the Test
Fixed cells are permeabilized, allowing conjugated antibodies access to proteins within the cell. Briefly vortex the tube and incubate for 30 - 45 minutes at room temperature in the dark. The conjugated antibody is allowed to penetrate and bind to its target within the cell. Harvest cells, and wash twice in cold HBSS or PBS by spinning at 200 x g for 7 minutes.