【产品名称】CD326 (EpCAM) Tumor Cell Enrichment and Detection Kit, human
The CD326 (EpCAM) Tumor Cell Enrichment and Detection Kit has been developed for the highly sensitive enrichment and detection of disseminated epithelial tumor cells from peripheral blood or bone marrow.
Detailed separation procedure
First, cells are labeled with CD326 (EpCAM) MicroBeads and enriched on the first MACS Column. Secondly, CD326+ cells are fixed, applied to a second MACS Column, and subjected to intracellular staining of the cytokeratin filaments using two alkaline phosphatase–conjugated cytokeratin-specific antibodies, clones CK3-11D5 and CK3-3E4. After elution from the column, cells are transferred onto a microscope slide, incubated with the substrate, and then analyzed by light microscopy. During the entire staining procedure, the cells remain retained within the column, thus minimizing cell loss.