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 植酸/总磷量检测试剂盒
  • 植酸/总磷量检测试剂盒
  • 植酸/总磷量检测试剂盒

    参考价格: ¥0.00
    产品型号:JKY/K-PHYT
    所在地区:北京
    上架时间:2012年09月28日
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    植酸/总磷量检测试剂盒
    名称:植酸/总磷量检测试剂盒
    产品货号:K-PHYT
    规格:50
    产地:爱尔兰
    数量:大量

    英文名:Phytic Acid/Total Phosphorus Assay Kit
    保存条件:4-6摄氏度
    保质期:1年以上



    外观四个玻璃小瓶和两个60毫升聚丙烯瓶

    比重 不适用
    在水中的溶解度易溶
    pH值 7.0
    气味 无
    粉末和液体形式

    稳定性 稳定 十年或以上(冷冻机)

    成分:
    名称 比例
    醋酸钠缓冲液60毫升 一小瓶(聚丙烯)
    甘氨酸缓冲液 一瓶(聚丙烯)
    植酸酶悬浮液 一个七毫升小瓶(玻璃)
    碱性磷酸酶悬浮液 一个七毫升小瓶(玻璃)
    磷标准溶液25毫升 一小瓶(玻璃)
    燕麦粉的控制液 一二毫升小瓶(玻璃)


    试剂盒:
    试剂盒适合执行50个化验可从
    包含完整的检测方法,另加:
    瓶1:醋酸钠缓冲液(25毫升,200毫米,pH值5.5)和
    叠氮化钠(0.02%瓦特/视频作为防腐剂)。
    稳定在4 ° C的“2年
    瓶2:植酸酶暂停(1.2毫升,12000单位/毫升)。
    稳定在4 ° C的“2年
    瓶3:甘氨酸缓冲液(25毫升,400毫米,pH值10.4),加氯化镁
    (4毫米),硫酸锌(0.4毫米)和叠氮化钠
    (0.02%瓦特/ 5)作为防腐剂。
    稳定在4 ° C的“2年
    瓶4:暂停碱性磷酸酶(1.2毫升,80单位/毫升)。
    稳定在4 ° C的“2年
    瓶5:磷标准溶液(24毫升,50微克/毫升)。
    和叠氮化钠(0.02%瓦特/视频作为防腐剂)。
    稳定在4 ° C的“2年
    瓶6:燕麦粉控制粉(5克,磷含量,
    见瓶)。
    稳定“在室温下5年。

    Phytic Acid / Total Phosphorus
    A simple method for the measurement of phytic acid / total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.

    Catalogue Number: K-PHYT
    Content: 50 assays

    Appearance Four glass vials and two 60 mL polypropylene containers.
    Specific Gravity Not applicable
    Solubility in Water Readily soluble.
    pH Value 7.0
    Odour none
    Form powders and liquids
    Stability stable in a freezer for ten or more years
    Ingredients Name CAS Proportion
    Sodium acetate buffer One 60 ml vial (polypropylene)
    Glycine Buffer One container (polypropylene)
    Phytase suspension One 7 ml vial (glass)
    Alkaline phosphatase suspension One 7 ml vial (glass)
    Phosphorus standard solution One 25 ml vial (glass)
    Oat flour control One 12 ml vial (glass)


    KITS:
    Kits suitable for performing 50 assays are available.The
    kits contain the full assay method plus:
    Bottle 1: Sodium acetate buffer (25 mL, 200 mM, pH 5.5) and
    sodium azide (0.02 % w/v) as a preservative.
    Stable for > 2 years at 4°C.
    Bottle 2: Phytase suspension (1.2 mL, 12,000 U/mL).
    Stable for > 2 years at 4°C.
    Bottle 3: Glycine buffer (25 mL, 400 mM, pH 10.4), plus MgCl2
    (4 mM), ZnSO4 (0.4 mM) and sodium azide
    (0.02 % w/v) as a preservative.
    Stable for > 2 years at 4°C.
    Bottle 4: Alkaline phosphatase suspension (1.2 mL, 80 U/mL).
    Stable for > 2 years at 4°C.
    Bottle 5: Phosphorus standard solution (24 mL, 50 μg/mL).
    and sodium azide (0.02 % w/v) as a preservative.
    Stable for > 2 years at 4°C.
    Bottle 6: Oat flour control powder (5 g; Phosphorus content,
    see bottle).
    Stable for > 5 years at room temperature.


    INTRODUCTION:
    Phytic acid (phytate; myo-inositol 1,2,3,4,5,6, hexakisphosphate) is the
    primary source of inositol and storage phosphorus in plant seeds
    contributing ~70 % of total phosphorus. The abundance of phytic acid
    in cereal grains is a concern in the foods and animal feeds industries
    because the phosphorus in this form is unavailable to monogastric
    animals due to a lack of endogenous phytases; enzymes specific for
    the dephosphorylation of phytic acid. In addition, the strong chelating
    characteristic of phytic acid reduces the bioavailability of other
    essential dietary nutrients such as minerals (e.g. Ca2+, Zn2+, Mg2+,
    Mn2+, Fe2+/3+), proteins and amino acids.1 High phytic acid content
    feeds are generally supplemented with inorganic phosphate, however
    this causes increased faecal phosphate levels and subsequent
    eutrophication of waterways. Alternatively, supplementation with
    commercial phytases is becoming increasingly popular and reduces the
    requirement for inorganic phosphate supplementation as well as the
    associated environmental issues.
    Currently, there is no commercially available, simple, quantitative
    method for phytic acid and while such measurement is relatively
    complex the generally accepted AOAC Method 986.11 has
    limitations.2 For each individual analysis the method requires
    cumbersome anion-exchange purification and a major inherent
    assumption here is that only phytic acid is purified. While this
    assumption is viable for non-processed grains for which phytic acid
    comprises at least 97 % of total inositol phosphates, it is not viable for
    processed foods and feeds which can contain higher levels of some
    lower myo-inositol phosphate forms (i.e. IP3, IP4, IP5) that would coelute
    with phytic acid and contribute to overestimation of the phytic
    acid content.3,4
    Given the complexities of the purification and measurement of phytic
    acid separate from lower myo-inositol phosphate forms, Megazyme
    has developed a simple, quantitative method (K-PHYT) to measure
    total “available phosphorus” released from food and feed samples that
    is amenable to high numbers of samples and does not require tedious
    anion-exchange purification. This method involves acid extraction of
    inositol phosphates followed by treatment with a phytase that is
    specific for phytic acid (IP6) and the lower myo-inositol phosphate
    forms (i.e. IP2, IP3, IP4, IP5). Subsequent treatment with alkaline
    phosphatase ensures the release of the final phosphate from myoinositol
    phosphate (IP1) which is relatively resistant to the action of
    phytase. The total phosphate released is measured using a modified
    colorimetric method and given as grams of phosphorus per 100 g of
    sample material.5,6
    1
    Alkaline phosphatase will also release phosphate from
    monophosphate esters other than myo-inositol phosphate, however
    analyses of a broad spectrum of samples using K-PHYT indicated that
    phosphorus released from monophosphate esters other than myoinositol
    phosphate is small.