上海叶舟生物科技有限公司

 Nru I from Nocardia rubra
  • Nru I from Nocardia rubra
  • Nru I from Nocardia rubra

    参考价格: ¥1895.00
    品  牌:Roche
    产品型号:10776777001 (1,000 U (10 U/μl))
    所在地区:上海
    上架时间:2012年09月27日
    留言咨询
    与企业取得联系时请告知该信息获取自中国教育装备采购网
    我要分享
    详细说明

    Nru I from Nocardia rubra
    名称:原装供应| Nru I from Nocardia rubra
    货号:10776777001
    规格:1,000 U (10 U/µl)
    产地:Roche
    价格:1895
    Source: From Nocardia rubra
    Incubation temperature: +37°C
    Quality
    Sequence specificity: Nru I recognizes the sequence T*CG↓°CG*A and generates fragments with blunt ends. The amount of radioactivity released is calculated as a percentage of input radioactivity, then divided by the number of units of enzyme. That number is stated under "Exo" (on the product label).
    Product Description
    Heat inactivation: Nru I can be heat inactivated by incubating it for 15 minutes at +65°C.
    Compatible ends: Nru I ends are compatible with any blunt end.
    Isoschizomers
    Approx. 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Nru I for 4 h at 37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithioerythritol, pH approx. 7.5.
    Nru I is an isoschizomer of Spo I.
    Methylation sensitivity: Nru I is inhibited by overlapping dam methylation at the sites indicated (*) on the recognition sequence. Depending on its position, the presence of 5-methylcytosine may either inhibit (*) or have no effect (°).