【产品名称】Nitrotyrosine, mAb (NOY-7A5)
Application: Western Blot (use 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
Specificity: Recognizes nitrotyrosine-modified proteins.
Purity Detail: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Formulation: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
Reconstitution: Reconstitute with 1ml distilled water (15 min., RT).
Use/Stability: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
Handling: After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Avoid freeze/thaw cycles.
Long Term Storage: -20°C