【产品名称】IL?4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human
Interleukin-4 (IL-4) is predominantly secreted by activated CD4+ memory and effector TH2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T2 types of immune mechanisms can also be involved in immunologic disorders such as IgE-mediated allergy.
IL-4 Secretion Assay was used to isolate and analyze Tetanus Toxoid-specific1, allergen-specific2,5, and autoreactive7 T cells from PBMCs, as well as tumor-specific cells from T cell lines3. The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells4, and for detection of IL-4-secreting NKT cells6.
IL-4-secreting CD8+ T cells were isolated in combination with CD8 MultiSort MicroBeads.