【产品名称】IFN-γ Secretion Assay – Detection Kit (APC), human
Virus-specific T cells were investigated after stimulation with peptides or proteins derived from influenza virus1, CMV2,3, EBV4,5,6, HIV5,7,8,9,10, HBV11, and ADV24.
Virus-specific T cells were expanded invitro1,2,3,5,6,9 showing highly specific and very efficient killing of target cells and have been analyzed for TCR clonotypes8,10.
The IFN-γ Secretion Assay was used for the isolation and analysis of antigen-specific T cells from PBMCs after stimulation with Tetanus Toxoid1, minor histocompatibility antigens, and tumor antigens12,13,14,15. The assay was also used to purify and analyze tumor-specific T cells from T cell lines13,14,for the isolation of functional antigen-specific, IFN-γ-secreting T cells reacting to other tumor antigens, e.g. SSX14, CEA16, or HER217 from PBMCs or TILs (tumor infiltrating lymphocytes)14,18.
The IFN-γ Secretion Assay was used to counterstain peptide-MHC-tetramer-labeled Melan A-specific CD8+ T cells to analyze functionality of the tetramer-positive cells.12,15
The IFN-γ Secretion Assay was also used for isolation and functional characterization of allergen-specific T cells.21 Furthermore, the IFN-γ Secretion Assay was used for epitopemapping of MHC class II peptides.19
IFN-γ-secreting human NK cells were isolated using the IFN-γ Secretion Assay.20 IFN-γ Secretion Assay reagents were reported to cross-react with chimpanzee cells22 but not with rhesus macaque cells.
The IFN-γ Secretion Assay can also be used for two-color cytokine analysis9 and allows counterstaining of peptide- MHC-tetramer-labeled cells 12,15. It can also be combined with flow cytometric proliferation assays