名称：原装订购| hEotaxin Biot FKit
All Reagents: 2° - 8° C.
Receptor density is then determined by flow cytometric analysis using 488 nm wavelength laser excitation. Peripheral Blood Cells: Whole blood collected in heparinized tubes should be processed by standard Ficoll-Hypaque gradient separation techniques to isolate mononuclear cells. Cells expressing the specific cytokine receptors are fluorescently stained, with the intensity of staining directly proportional to the density of the receptors.
Cells should be resuspended to a final concentration of 4 x 106 cells/mL in 10 mM PBS. Designed to quantitatively determine the percentage of cells bearing cytokine receptors within a population and to estimate the receptor density for the human chemokine Eotaxin on cell surfaces by flow cytometry. Ficoll and contaminating serum components should be removed by washing the cells twice with 10 mM PBS and then resuspending the cells in 10 mM PBS.