上海叶舟生物科技有限公司

 hEotaxin Biot FKit
  • hEotaxin Biot FKit
  • hEotaxin Biot FKit

    参考价格: ¥5100.00
    品  牌:R&D
    产品型号:NFEO0 (1 KT)
    所在地区:上海
    上架时间:2012年09月27日
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    hEotaxin Biot FKit
    名称:原装订购| hEotaxin Biot FKit
    货号:NFEO0
    产地:R&D
    规格:1 KT
    价格:5100
    All Reagents: 2° - 8° C.
    Sample Preparation
    Receptor density is then determined by flow cytometric analysis using 488 nm wavelength laser excitation. Peripheral Blood Cells: Whole blood collected in heparinized tubes should be processed by standard Ficoll-Hypaque gradient separation techniques to isolate mononuclear cells. Cells expressing the specific cytokine receptors are fluorescently stained, with the intensity of staining directly proportional to the density of the receptors.
    Intended Use
    Cells should be resuspended to a final concentration of 4 x 106 cells/mL in 10 mM PBS. Designed to quantitatively determine the percentage of cells bearing cytokine receptors within a population and to estimate the receptor density for the human chemokine Eotaxin on cell surfaces by flow cytometry. Ficoll and contaminating serum components should be removed by washing the cells twice with 10 mM PBS and then resuspending the cells in 10 mM PBS.