名称：原装订购| Human CCL11/Eotaxin Quantikine ELISA Kit
Plasma - Collect plasma using citrate or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles.
Calibrator Diluent RD6O contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.
SAMPLE COLLECTION AND STORAGE
Cell Culture Supernates - Remove particulates by centrifugation and assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles. Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles.