名称：原装订购| hEpo Biot FKit
All Reagents: 2° - 8° C
Designed to quantitatively determine the percentage of cells expressing cytokine receptors within a population and to estimate the receptor density for the human Erythropoietin (EPO) receptor on cell surfaces by flow cytometry.
1X RDF1 Wash Buffer: Add 1 mL of 10X concentrate to 9 mL of sterile distilled water. Reagent should be maintained on ice during use and stored at 2° - 8° C for no more than 2 months.
Principle of the Test
The cells are then directly incubated with avidin-fluorescein, which attaches to the receptor-bound biotinylated cytokine. Biotinylated rhEPO: Add 1 mL of sterile distilled water to the lyophilized product.
Mix gently until the product is completely dissolved. Reagent yields optimal activity when used within 60 days of reconstitution. Washed cells are incubated with the biotinylated cytokine that in turn binds to the cells via specific cell surface receptors.