名称：原装订购| hFactor III/TF QKit
SAMPLE COLLECTION AND STORAGE
Cell Culture Supernates - Remove particulates by centrifugation and assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles.
The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, Human Coagulation Factor III Controls (optional; available from R&D Systems). and clothing protection when using this material. Cell Lysates - Cells must be lysed before assaying. Refer to the Sample Values section for an example.
Cell Lysis Buffer 1 contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
Plasma - Collect plasma using citrate, heparin, or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles.