名称：正品原装| Mouse IL-2 ELISpot Kit
Store the unopened kit at 2 - 8° C. Do not use beyond the kit expiration date. This kit is validated for single use only. Results obtained with opened/reconstituted reagents at a later date may not be reliable.
To minimize edge effect, place the microplate (bottom down) onto a piece of aluminum foil (about 4 x 6 inches). If crystals have formed in the concentrate, warm to room temperature and mix gentlyuntil the crystals have completely dissolved. The foil may be left on the microplate for the rest of the experimental procedure and removed after the BCIP/NBT has been washed off. Do not touch PVDF membrane filters with pipette tips when pipetting cells and reagents to avoid damage to the membranes.
After completion of the experiment, do not dry the microplate at a temperature higher than 37° C since it may cause cracking of the PVDF membrane filters. Some components of this kit contain sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Add cells, cover the microplate with the lid and shape the foil around the edges of the microplate. Flush with large volumes of water during disposal. Do not use reagents from this kit with components from other R&D Systems’ ELISpot or ELISA kits and/or components manufactured by other vendors.