名称：正品原装| Mouse Osteopontin (OPN) Quantikine ELISA Kit
Unopened Kit Store at 2 - 8° C. Do not use past kit expiration date.
SAMPLE COLLECTION AND STORAGE
Allow the plate to soak for at least 30 seconds between washes to improve assay performance. Serum - Allow blood samples to clot for 2 hours at room temperature or overnight at 2 - 8° C before centrifuging. Centrifuge for 20 minutes at 2000 x g. Remove serum and assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles. It is recommended that the samples be pipetted within 15 minutes.
When mixing or reconstituting protein solutions, always avoid foaming. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Cell Culture Supernates - Remove particulates by centrifugation and assay immediately or aliquot and store samples at ≤-20° C. Avoid repeated freeze-thaw cycles. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.