名称：正品原装| Phos ERK1/ERK2 Cell-Bas ELISA
TECHNICAL HINTS AND LIMITATIONS
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. This Cell-Based ELISA should not be used beyond the expiration date on the kit label. This Cell-Based ELISA contains the components required to run an ELISA using fluorogenic substrates to measure dually phosphorylated ERK1 and ERK2 in whole cells. This may interfere with the sensitivity of the assay. Buffers containing protein should be made under aseptic conditions and stored at 2-8° C.
PRINCIPLE OF THE ASSAY
When mixing or reconstituting protein solutions, always avoid foaming. These kinases are approximately 85% homologous and share identical amino acid sequences surrounding the phosphorylation sites T202/Y204 on ERK1 and T185/Y187 on ERK2. Do not mix or substitute reagents with those from other lots or sources. Avoid microbial contamination of reagents and buffers.