名称：正品订购| Human CXCR1/IL-8 RA Fluorescein MAb (Clone 42705), Mouse IgG2A
Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C. Washed cells are incubated with the fluorescein-labeled monoclonal antibody, which binds to the cells expressing the IL-8 RA. Whole blood should be collected in evacuated tubes containing EDTA or heparin as the anti-coagulant.
Continuous cell lines or activated cell cultures should be centrifuged at 500 x g for 5 minutes and washed three times in an isotonic PBS buffer (supplemented with 0.5% BSA), Designed to quantitatively determine the percentage of cells bearing the cell surface receptor for human IL-8 (IL-8 RA, CXCR-1) within a population and qualitatively determine the density of this receptor on cell surfaces by flow cytometry. Cell surface expression of the IL-8 RA is determined by flow cytometric analysis using 488 nm wavelength laser excitation.