名称：正品订购| Mouse IL-2 Phycoerythrin MAb (Clone JES6-5H4), Rat IgG2B
Storage: Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C. For intracellular staining, the cells must first be fixed and permeabilized.
The use of 4% paraformaldehyde in PBS as a fixative is recommended. The reagent is designed for flow cytometric applications intended to identify and quantitate cells possessing cytoplasmic forms of the protein recognized by the monoclonal.
Principle of the Test
Fixed cells are permeabilized, allowing conjugated antibodies access to proteins within the cell. Cells are initially fixed in order to minimize leakage of proteins out of the cell. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used in both the staining as well as washing steps. The conjugated antibody is allowed to penetrate and bind to its target within the cell. Centrifuge the cells at 200 x g for 7 minutes. Following a final wash, the cells are analyzed on a flow cytometer.