名称：原装订购| Recombinant Porcine TNF-alpha, CF
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. Measured in a cytotoxicity assay using TNFsusceptible PK-15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D.
3 months, 20 to 70 °C under sterile conditions after reconstitution.
12 months from date of receipt, 20 to 70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method. Upon receipt, store it immediately at the temperature recommended below.
Purity >97%, by SDSPAGE under reducing conditions and visualized by silver stain. Formulation Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness . Source E. coliderived.